Furan oxidation based cross-linking: a new approach for the ... - PubMed
网页2016年1月28日 · Furan mediated nucleic acid cross-linking, initially developed for DNA interstrand duplex cross-linking, has matured into a versatile tool for the study of protein and nucleic acid interactions, ready to face its applications. The methodology was initially developed for easy and clean chemical gener ….
Revealed pathways of furan ring opening and surface crosslinking …
网页2023年4月3日 · Poly (furfuryl alcohol) – (PFA) is a biobased thermoset known for its highly crosslinked structure. Bidimensional NMR (2D-NMR) was employed to give new insights on the complex structural organization in PFA with a special emphasis on the side structures resulting from furan ring-opening.
Crosslinker-modified nucleic acid probes for improved target ...
网页By covalently fixing this interaction through activation of a furan crosslinker, more selective and higher yielding in vitro ncRNA target identification should become possible. Recent studies in our group focus on the use of furan crosslinking-enhanced enrichment of lncRNA sequences in vitro.
Chemical RNA Cross-Linking: Mechanisms, Computational …
网页2023年1月16日 · Cross-link-ligation methods have a wide range of applications in RNA biology and medicine. The earliest applications of these chemical tools were critical in establishing the structural mechanisms of pre-mRNA splicing and rRNA processing.
Cell Surface Biotinylation Using Furan Cross-Linking Chemistry
网页We here detail a new surface biotinylation protocol based on furan-biotin affinity purification to enrich plasma membrane proteins for proteomics. This protocol involves biotinylation of cell surface membrane proteins on viable cells, followed by affinity enrichment using streptavidin beads, trypsin digestion, peptide cleanup, and LC-MS/MS ...
Furan-PNA: a mildly inducible irreversible interstrand crosslinking ...
网页2016年4月27日 · We here report on the design and synthesis of tailor-made furan-modified peptide nucleic acid (PNA) probes for covalent targeting of single stranded DNA through a crosslinking strategy. After introducing furan-containing building blocks into a PNA sequence, hybridization and furan-oxidation based crosslinking to DNA is investigated.
Furan-based (photo)oxidation reactions and their application in …
网页2023年10月1日 · Furan is used as a pro-reactive chemical handle to covalently capture an ODN target. •. The electrophilic moiety that is generated in situ can react with naturally occurring targets. •. DNA-DNA, DNA-RNA or DNA-Peptide crosslinking can be achieved with careful design of the probes. •.
Synthesis and Improved Cross-Linking Properties of C5-Modified Furan …
网页In this work, we combined both strategies to fine-tune PNA crosslinking towards single stranded DNA sequences using a furan oxidation-based crosslinking method; for this purpose, γ- l -lysine and γ- l -arginine furan-PNA monomers were synthesized and incorporated in PNA sequences via solid phase synthesis.
Revealed pathways of furan ring opening and surface crosslinking …
网页2023年4月3日 · Poly (furfuryl alcohol) – (PFA) is a biobased thermoset known for its highly crosslinked structure. Bidimensional NMR (2D-NMR) was employed to give new insights on the complex structural organization in PFA with a special emphasis on the side structures resulting from furan ring-opening.
Furan oxidation based cross-linking: a new approach for the study …
网页This group has developed a cross-link strategy where a furan moiety is incorporated into oligodeoxynucleotides (ODNs), which display an unprecedented selectivity for cross-linking toward a cytidine opposite the modified residue, forming one specific cross-linked duplex, which could be isolated in good yield.
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