N-Glycans - Essentials of Glycobiology - NCBI Bookshelf
网页Hybrid N-glycans are formed if the GlcNAcMan 5 GlcNAc 2 glycan produced by MGAT1 is not acted on by α-mannosidase II. Incomplete action of α-mannosidase II can result in GlcNAcMan 4 GlcNAc 2 hybrids.
An N- glycome tissue atlas of 15 human normal and cancer
网页2024年1月4日 · N- glycosylation is an abundant post-translational modification of most cell-surface proteins. N- glycans play a crucial role in cellular functions like protein folding, protein localization,...
A library of chemically defined human N-glycans synthesized from ...
网页2017年11月21日 · Armed with these and other commercially available GTs, we efficiently transformed precursor oligosaccharides into a library of hybrid and complex N-glycans including asymmetric...
Global site-specific analysis of glycoprotein N-glycan processing
网页2017年3月28日 · This protocol describes a semiquantitative glycoproteomics method using sequential treatment with endoglycosidases to create unique mass signatures to determine glycan occupancy and proportion of...
Recent Advances in the Chemical Biology of N -Glycans
网页2021年2月16日 · Hybrid N-glycans have both high-mannose and complex-type structures. Thus, N-glycans have diverse structures and are involved in a variety of biological phenomena. However, the molecular bases of their modes of …
Cell engineering for the production of hybrid-type N-glycans …
网页2021年9月22日 · Glycan structural analysis revealed that complex-type N-glycans were removed in both M2D-KO and DF-KO cells. Our results suggest that these cell lines are suitable for the production of therapeutic proteins with hybrid-type N-glycans.
Predominant Expression of Hybrid N-Glycans Has Distinct …
网页2016年6月13日 · This newly engineered cell line enabled us to compare N-glycan effects on cellular properties of hybrid type N-glycans, to the well-established Pro − 5 and Lec1 cell lines, which express complex and oligomannose types of N-glycans, respectively. Lectin binding studies revealed the predominant N-glycan expressed
Predominant Expression of Hybrid N-Glycans Has Distinct …
网页2016年6月13日 · This newly engineered cell line enabled us to compare N-glycan effects on cellular properties of hybrid type N-glycans, to the well-established Pro−5 and Lec1 cell lines, which express complex and oligomannose types of N-glycans, respectively. Lectin binding studies revealed the predominant N-glycan expressed in K16 is hybrid type.
N-Glycan Biosynthesis: Basic Principles and Factors ... - Springer
网页2021年10月23日 · High-mannose type N-glycan is characterized by having not undergone any processing in the Golgi compartment. Hybrid-type N-glycan typically has only one branch that has been processed in the Golgi. Bulky complex-type N-glycans in turn have two to five branches that are made and terminated in the medial and trans-Golgi cisternae
N-Glycan Branching and Its Biological Significance
网页2014年1月1日 · N-glycan, an Asn-linked oligosaccharide found on glycoproteins, is structurally and functionally diverse. N-glycans are roughly classified into three groups – high-mannose-, hybrid-, and complex-type glycans – according to the structures at their nonreducing termini (Stanley et al. 2009).