Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages
网页2018年10月19日 · Here we describe phagocytic assays, using time-lapse spinning disk confocal microscopy, that allow 3D imaging of single phagocytic events. In addition, we describe assays to unambiguously image Fcγ receptor- …
High-resolution quantification of discrete phagocytic events by …
网页2020年3月3日 · We present a live-cell, time-lapse and high-content microscopy imaging method based on the detection and quantification of fluorescent dye ‘voids’ within phagocytes that result from target internalization to quantify phagocytic events with high temporal resolution.
Identification of phagocytosis regulators using magnetic ... - Nature
网页2018年11月5日 · These orthogonal time-lapse microscopy validation assays demonstrated varying degrees of reduced or increased phagocytic capacity across multiple substrates, largely mirroring the original screen...
Targeted macrophage phagocytosis by Irg1/itaconate axis
网页Here we explore whether itaconate regulates phagocytosis. Methods: Phagocytosis of macrophages was investigated by time-lapse video recording, flow cytometry, and immunofluorescence staining in macrophage/microglia cultures isolated from mouse tissue. Unbiased RNA-sequencing and ChIP-sequencing assays were used to explore the underlying mechanisms.
Phagocytosis Dynamics Depends on Target Shape - PMC
网页2013年9月3日 · Time-lapse images from a typical phagocytosis event to illustrate the start and end points of phagocytosis. Only those frames that were in focus during z-stack data acquisition are shown here. The timestamps on the frames indicate the time elapsed since the start of image acquisition ( t = 0).
Macrophage phagocytosis assay with reconstituted target …
网页2020年6月19日 · To study how phagocytosis is affected by specific changes to the target surface, we developed an in vitro assay based on reconstituted membrane-coated target particles to which known molecules...
Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages
网页2018年10月19日 · Watch this Scientific Journal Video about Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages at JoVE.com
网页Here we describe phagocytic assays, using time-lapse spinning disk confocal microscopy, that allow 3D imaging of single phagocytic events. In addition, we describe assays to unambiguously image Fcγ receptor-
Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages
网页2018年10月19日 · Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy
网页Macrophage phagocytosis can be triggered by diverse receptor-ligand interactions to clear pathogens and dead cells from a host. Many ways of assaying phagocytosis exist that...