Cellosaurus cell line CT-2A (CVCL_ZJ44)
WEBCellosaurus CT-2A (CVCL_ZJ44) Characteristics: Mouse model for therapeutic research on brain malignancies (Millipore=SCC194). Characteristics: Established from a tumor maintained through serial intracranial transplants. Derived from site: In situ; Brain; UBERON= UBERON_0000955. Breed/subspecies: C57BL/6J.
Immune phenotyping of diverse syngeneic murine brain tumors …
WEBAug 6, 2020 · We utilized tumor cells from two chemically induced tumor models, CT2A and GL261, and three GEMM-derived cell lines, 005, Mut3, and Mut4 that are genetically distinct.
Cellosaurus cell line CT-2A-Luc (CVCL_ZJ60)
WEBResource Identification Initiative. To cite this cell line use: CT-2A-Luc (RRID:CVCL_ZJ60) Comments. Characteristics: Transduced with a lentiviral vector containing a Fluc-IRES-GFP construct expressed under the control of the CMV promoter.
Stemness of the CT-2A Immunocompetent Mouse Brain Tumor …
WEBApr 12, 2012 · CT-2A expression of the BTSC marker, CD133, increased from 2% in monolayer cells to 31% in fully-formed neurospheres. Investigation of three stem cell markers (Oct4, Nanog and Nestin) revealed a distinct stemness signature with monolayer cells expressing Oct4 and Nestin (no Nanog), and neurospheres expressing all three.
CT-2A neurospheres-derived high-grade glioma in mice: a new …
WEBSep 9, 2019 · The murine CT-2A cell line was established in C57BL/6J mice by Seyfried and colleagues in 1992 (Seyfried et al., 1992). Monolayer(ML)-cultured CT-2A (ML/CT-2A) cells implanted in immune-competent C57BL/6J mice give rise to orthotopic HGGs (Martinez-Murillo and Martinez, 2007).
Treatment of an aggressive orthotopic murine glioblastoma model …
WEBMar 5, 2020 · Of the 29 CT2A neoantigens screened, we identified neoantigen-specific CD8+ T-cell responses in the intracranial TIL and draining lymph nodes to two H2-K b restricted (Epb4 H471L and Pomgnt1 R497L) and one H2-D …
Immunotherapy with subcutaneous immunogenic autologous …
WEBOct 24, 2017 · In the current study we used two fully immunocompetent glioma mouse models: the standard GL261 model and the CT2A model. Both are syngeneic cell lines for C57BL/6 mice but originate from...
Conjugation of glucosylated polymer chains to checkpoint …
WEBOct 11, 2021 · Inspired by the successful anticancer effect against the GL261 GBM model, we further applied Gluc25-S-aPD-L1 for the treatment of CT2A cell line, which presents lower expression of phosphatase...
CT-2A neurospheres-derived high-grade glioma in mice: a new ... - PubMed
WEBSep 12, 2019 · To address this need, we developed and comprehensively characterized a new in vivo model based on the orthotopic implantation of CT-2A cells cultured in neurospheres (NS/CT-2A). Murine CT-2A methylcholanthrene-induced HGG cells (C57BL/6 background) were cultured in monolayers (ML) or NS and orthotopically inoculated in syngeneic animals.
CRISPR-Cas knockout of miR21 reduces glioma growth - Cell Press
WEBJun 16, 2022 · Sanger sequencing and next-generation sequencing (NGS) were used to validate the CRISPR activity and to verify the miR-21 KO in mouse (GL261and CT2A) and human (U87) glioma lines (Figure 1 A). In detail, a CRISPR-Cas genome-editing system was used to KO miR-21 gene expression, as illustrated in Figure S1 A.
- Some results have been removed